Published In
PLoS ONE
Document Type
Article
Publication Date
5-8-2013
Subjects
Genome sequencing -- Techniques -- Evaluation, Spiders -- Genome mapping -- Analysis, Phylogenetics -- Effect of technological innovations on, Polymerase chain reaction -- Methodology
Abstract
The development of second generation sequencing technology has resulted in the rapid production of large volumes of sequence data for relatively little cost, thereby substantially increasing the quantity of data available for phylogenetic studies. Despite these technological advances, assembling longer sequences, such as that of entire mitochondrial genomes, has not been straightforward. Existing studies have been limited to using only incomplete or nominally intra-specific datasets resulting in a bottleneck between mitogenome amplification and downstream high-throughput sequencing. Here we assess the effectiveness of a wide range of targeted long-range PCR strategies, encapsulating single and dual fragment primer design approaches to provide full mitogenomic coverage within the Araneae(Spiders).
DOI
10.1371/journal.pone.0062404
Persistent Identifier
http://archives.pdx.edu/ds/psu/9927
Citation Details
Briscoe AG, Goodacre S, Masta SE, Taylor MI, Arnedo MA, et al. (2013) Can Long-Range PCR Be Used to Amplify Genetically Divergent Mitochondrial Genomes for Comparative Phylogenetics? A Case Study within Spiders (Arthropoda: Araneae). PLoS ONE 8(5): e62404.
Description
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© 2013 Briscoe et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.