This work was supported by the National Institutes of Health (SC2-GM113727 and F32-GM090492 to J.A.R., R01- GM087628 to D.R.D. and S.E.); and the National Science Foundation (MCB-1330427 to S.E. and D.R.D.).
Molecular Biology and Evolution
Caenorhabditis, Mitochondrial DNA, Variation (Biology)
To study mitochondrial-nuclear genetic interactions in the nematode Caenorhabditis briggsae, our three laboratories independently created 38 intra-species cytoplasmic-nuclear hybrid (cybrid) lines. Although the cross design combines maternal mitotypes with paternal nuclear genotypes, eight lines (21%) unexpectedly contained paternal mitotypes. All eight share in common ancestry of one of two genetically related strains. This unexpected parallel observation of paternal mitochondrial transmission, undesirable given our intent of creating cybrids, provides a serendipitous experimental model and framework to study the molecular and evolutionary basis of uniparental mitochondrial inheritance.
Ross JA, Howe DK, Coleman-Hulbert A, Denver DR, Estes S. (2016). Paternal Mitochondrial Transmission in Intra-Species Caenorhabditis briggsae Hybrids. Molecular Biology and Evolution.