Title

A Phage Display-Selected Peptide Inhibitor o fAgrobacterium Vitis Polygalacturonase

Published In

Molecular Plant Pathology

Document Type

Citation

Publication Date

4-21-2016

Abstract

Agrobacterium vitis, the causal agent of crown gall of grapevine,is a threat to viticulture worldwide. A major virulence factor of thispathogen is polygalacturonase, an enzyme that degrades pectincomponents of the xylem cell wall. A single gene encodes for thepolygalacturonase gene. Disruption of the polygalacturonase generesults in a mutant that is less pathogenic and produces signifi-cantly fewer root lesions on grapevines. Thus, the identification ofpeptides or proteins that could inhibit the activity ofpolygalacturonase could be part of a strategy for the protection ofplants against this pathogen. A phage-displayed combinatorialpeptide library was used to isolate peptides with a high bindingaffinity to A. vitis polygalacturonase. These peptides showedsequence similarity to regions of Oryza sativa (EMS66324,Japonica) and Triticum urartu (NP_001054402, wild wheat)polygalacturonase-inhibiting proteins (PGIPs). Furthermore, thesepanning experiments identified a peptide, SVTIHHLGGGS, whichwas able to reduce A. vitis polygalacturonase activity by 35%in vitro. Truncation studies showed that the IHHL motif alone issufficient to inhibit A. vitis polygalacturonase activity.

Locate the Document

http://doi.org/10.1111/mpp.12293.

DOI

10.1111/mpp.12293

Persistent Identifier

http://archives.pdx.edu/ds/psu/18893

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