A Phage Display-Selected Peptide Inhibitor o fAgrobacterium Vitis Polygalacturonase
California Department of Food and Agriculture UC Pierce's Disease Research Grant Program
Molecular Plant Pathology
Agrobacterium vitis, the causal agent of crown gall of grapevine,is a threat to viticulture worldwide. A major virulence factor of thispathogen is polygalacturonase, an enzyme that degrades pectincomponents of the xylem cell wall. A single gene encodes for thepolygalacturonase gene. Disruption of the polygalacturonase generesults in a mutant that is less pathogenic and produces signiﬁ-cantly fewer root lesions on grapevines. Thus, the identiﬁcation ofpeptides or proteins that could inhibit the activity ofpolygalacturonase could be part of a strategy for the protection ofplants against this pathogen. A phage-displayed combinatorialpeptide library was used to isolate peptides with a high bindingafﬁnity to A. vitis polygalacturonase. These peptides showedsequence similarity to regions of Oryza sativa (EMS66324,Japonica) and Triticum urartu (NP_001054402, wild wheat)polygalacturonase-inhibiting proteins (PGIPs). Furthermore, thesepanning experiments identiﬁed a peptide, SVTIHHLGGGS, whichwas able to reduce A. vitis polygalacturonase activity by 35%in vitro. Truncation studies showed that the IHHL motif alone issufﬁcient to inhibit A. vitis polygalacturonase activity.
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Warren, J. G., Kasun, G. W., Leonard, T., & Kirkpatrick, B. C. (2016). A phage display-selected peptide inhibitor of Agrobacterium vitis polygalacturonase. Molecular Plant Pathology,17(4), 480–6.