Published In

FEMS Microbiology Letters

Document Type

Article

Publication Date

6-6-2011

Subjects

Archaebacteria -- Genetics, Gene expression, RNA, Ribosomes

Abstract

Ribosomal genes are strongly regulated dependent on growth phase in all organisms, but this regulation is poorly understood in Archaea. Moreover, very little is known about growth phase-dependent gene regulation in Archaea. SSV1- based lacS reporter gene constructs containing the Sulfolobus 16S/23S rRNA gene core promoter, the TF55a core promoter, or the native lacS promoter were tested in Sulfolobus solfataricus cells lacking the lacS gene. The 42-bp 16S/23S rRNA gene and 39-bp TF55a core promoters are sufficient for gene expression in S. solfataricus. However, only gene expression driven by the 16S/23S rRNA gene core promoter is dependent on the culture growth phase. This is the smallest known regulated promoter in Sulfolobus. To our knowledge, this is the first study to show growth phase-dependent rRNA gene regulation in Archaea.

Description

Copyright 2011 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved.

This article was archived with author permission and published as Open Access. Link to original instance.

DOI

10.1111/j.1574-6968.2011.02313.x

Persistent Identifier

http://archives.pdx.edu/ds/psu/9930

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