Sponsor
We gratefully acknowledge the financial support provided by the National Institutes of Health (CA127622 to B.S.M. and AI51629 to K.A.R.) and the Life Sciences Research Foundation via a Tularik postdoctoral fellowship to A.S.E.
Document Type
Post-Print
Publication Date
8-2010
Subjects
Antineoplastic agents, Marine bacteria, Polyketides -- Synthesis, Polyketides -- Metabolism
Abstract
A new series of coenzyme A-tethered polyketide synthase extender units were discovered in relation to the biosynthesis of the salinosporamide family of anticancer agents from the marine bacterium Salinispora tropica. In vivo and in vitro experiments revealed that the crotonyl-CoA reductase/carboxylase SalG has broad substrate tolerance toward 2-alkenyl-CoAs that give rise to the salinosporamide C-2 substitution pattern.
DOI
10.1021/ja9042824
Persistent Identifier
http://archives.pdx.edu/ds/psu/19065
Citation Details
Liu, Yuan; Hazzard, Christopher; Eustáquio, Alessandra S.; Reynolds, Kevin A.; and Moore, Bradley S., "Biosynthesis of Salinosporamides from α,β-Unsaturated Fatty Acids: Implications for Extending Polyketide Synthase Diversity" (2010). Chemistry Faculty Publications and Presentations. 169.
http://archives.pdx.edu/ds/psu/19065
Description
Supporting Information Available: Experimental procedures, NMR data and SalG kinetic data. This material is available free of charge via the Internet at http://pubs.acs.org.
This is the author’s version of a work that was accepted for publication in the Journal of the American Chemical Society. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of the American Chemical Society, 2009, 131 (30), pp 10376–10377 and can be found online at: https://doi.org/10.1021/ja9042824