Journal of the Optical Society of America A: Optics, Image Science and Vision
Microscopy -- Technique -- Innovations, Image processing -- Digital techniques, Microscopes -- Calibration
We present a method of using an unmodified differential interference contrast microscope to acquire quantitative information on scatter and absorption of thin tissue samples. A simple calibration process is discussed that uses a standard optical wedge. Subsequently, we present a phase-stepping procedure for acquiring phase gradient information exclusive of absorption effects. The procedure results in two-dimensional maps of the local angular (polar and azimuthal) ray deviation. We demonstrate the calibration process, discuss details of the phase-stepping algorithm, and present representative results for a porcine skin sample.
Duncan, D. D., Fischer, D. G., Dayton, A., & Prahl, S. A. (2011). Quantitative Carre differential interference contrast microscopy to assess phase and amplitude. [Article]. Journal of the Optical Society of America a-Optics Image Science and Vision, 28(6), 1297-1306.