In Vivo Quantification of Reactive Oxygen Species Demonstrates High Levels of Oxidative Stress in Base Excision Repair-Deficient Caenorhabditis Elegans: Implications for Associative Metabolic Phenotypes
Date of Award
Suzanne R. Estes
DNA repair, Mitochondrial DNA, Metabolic syndrome
In this present study, I use C. elegans as a model organism to better characterize the phenotypic response to deficiencies in base excision repair (BER). This will help to elucidate how DNA damage, increased mutagenesis, and exposure to reactive oxygen species (ROS) is related to Metabolic Syndrome. Additionally, this work will establish a starting point for investigation that can further characterize phenotypic responses such as mitochondrial physiology and morphology through measurements of mitochondrial membrane potential and mitochondrial circularity. The overall goal of this research is to better understand the nature of mitochondrial dysfunction with respect to the inactivation of DNA glycosylases, as well as understand the contribution DNA repair processes have to the maintenance of energy homeostasis and substrate oxidation.
Hase, Travis Lee, "In Vivo Quantification of Reactive Oxygen Species Demonstrates High Levels of Oxidative Stress in Base Excision Repair-Deficient Caenorhabditis Elegans: Implications for Associative Metabolic Phenotypes" (2013). University Honors Theses. Paper 10.