This work was funded by grants from the National High Technology Research and Development Program of China (863 Program) (Grant No. 2011AA100606), the Important Projects in the Scientific Innovation of Colleges and Universities in China (Grant No.70S018), the National Natural Science Foundation of China (Grant No. 30971804, 31101091, 31101172), the New Century Excellent Talents in University (Grant No. NCET-08-0693) and the Excellent Innovation Team Project of Jilin Province, China (Grant No.20111815). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Genetics -- Transcription, Safflower, Oleosins, Flavonoids, Biosynthesis
Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid biosynthesis and metabolism in safflower.
Li, Haiyan, et al. "De novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids." PloS one 7.2 (2012)