Neomycin: An Effective Inhibitor of Jasmonate-Induced Reactions in Plants
German Academic Exchange Service (DAAD; PPP Project ID 57136171) to AM, Funding of the National Science Foundation (NSF) (Grants IOS 1457369 and 1656057) to DJB
Journal of Plant Growth Regulation
Jasmonates are important phytohormones involved in both plant developmental processes as well as defense reactions. Many JA-mediated plant defense responses have been studied in model plants using mutants of the jasmonate signaling pathway. However, in plant species where JA-signaling mutants are not accessible, the availability of a tool targeting JA signaling is crucial to investigate jasmonate-dependent processes. Neomycin is a poly-cationic aminoglycoside antibiotic that blocks the release of Ca2+ from internal stores. We examined the inhibitory activities of neomycin on different jasmonate-inducible responses in eight different plant species: Intracellular calcium measurements in Nicotiana tabacum cell culture, Sporamin gene induction in Ipomoea batatas, PDF2.2 gene expression in Triticum aestivum, Nepenthesin protease activity measurement in Nepenthes alata, extrafloral nectar production in Phaseolus lunatus, nectary formation in Populus trichocarpa, terpene accumulation in Picea abies, and secondary metabolite generation in Nicotiana attenuata. We are able to show that neomycin, an easily manageable and commercially available compound, inhibits JA-mediated responses across the plant kingdom.
Locate the Document
Vadassery, J., Ballhorn, D. J., Fleming, S. R., Mazars, C., Pandey, S. P., Schmidt, A., ... & Mithöfer, A. (2019). Neomycin: an effective inhibitor of jasmonate-induced reactions in plants. Journal of Plant Growth Regulation, 38(2), 713-722.