Sponsor
This study was funded by the European Union program MaCuMBA (grant agreement 311975) and by the US-National Science Foundation (OCE1235432 and OCE 1558795.
Published In
Systematic and Applied Microbiology
Document Type
Post-Print
Publication Date
5-1-2019
Subjects
Hydrothermal vents, Thermophilic bacteria -- Genomics, Thermophilic bacteria -- Phylogeny -- Molecular aspects
Abstract
A novel moderately thermophilic, heterotrophic anaerobe, designated strain LG1T, was isolated from the Mariner deep-sea hydrothermal vent field along the Eastern Lau Spreading Center and Valu Fa Ridge. Cells of strain LG1T were motile rods, occurring singly or in pairs, 0.6μm in width and 1.2μm in length. The strain LG1T grew between 40 and 70°C (optimum 50-55°C), at a pH between 5 and 8 (optimum pH 6.5) and with 7.5-50gL-1 NaCl (optimum 30gL-1). Sulfur, cystine and thiosulfate were reduced to sulfide, and cell yield was improved in the presence of cystine. Strain LG1T was an organotroph able to use a variety of organic compounds. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain LG1T was affiliated to the genus Marinitoga within the order Petrotogales. It shared 95.34-96.31% 16S rRNA gene sequence similarity with strains of other Marinitoga species, and is most closely related to Marinitoga okinawensis. Genome analysis revealed the presence of a prophage sharing high sequence homology with the viruses MPV1, MCV1 and MCV2 hosted by Marinitoga strains. Based on the data from the phylogenetic analyses and the physiological properties of the novel isolate, we propose that strain LG1T is a representative of a novel species, for which the name Marinitoga lauensis sp. nov. is proposed; the type strain is LG1T (=DSM 106824=JCM 32613).
Rights
© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
DOI
10.1016/j.syapm.2019.02.006
Persistent Identifier
https://archives.pdx.edu/ds/psu/29944
Citation Details
L'Haridon, Stéphane; Gouhie, Léna; St. John, Emily; and Reysenbach, Anna-Louise, "Marinitoga Lauensis sp. nov., A Novel Deep-sea Hydrothermal Vent Thermophilic Anaerobic Heterotroph with a Prophage" (2019). Biology Faculty Publications and Presentations. 274.
https://archives.pdx.edu/ds/psu/29944
Description
This is the author’s version of a work. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Systematic and Applied Microbiology, 42(3), 343-347.