Sponsor
This work was supported by the grants NSF-PEET DEB-0328326 and NSF-MCB-0084224 awarded to A.-L. R. S. L. C. was supported by the OSU-PSU IGERT program, NSF-0114427.
Published In
International Journal of Systematic and Evolutionary Microbiology
Document Type
Post-Print
Publication Date
2010
Subjects
Thermophilic bacteria -- Costa Rica, Water -- Microbiology, Hot springs -- Costa Rica, Thermophilic bacteria -- Phylogeny
Abstract
A thermophilic bacterium, designated strain CR11T , was isolated from a filamentous sample collected from a terrestrial hot spring on the south-western foothills of the Rincón volcano in Costa Rica. The Gram-negative cells are approximately 2.4–3.9 mm long and 0.5–0.6 mm wide and are motile rods with polar flagella. Strain CR11T grows between 65 and 85 6C (optimum 75 6C, doubling time 4.5 h) and between pH 4.8 and 7.8 (optimum pH 5.9–6.5). The isolate grows chemolithotrophically with S0 , S2O2{ 3 or H2 as the electron donor and with O2 (up to 16 %, v/v) as the sole electron acceptor. The isolate can grow on mannose, glucose, maltose, succinate, peptone, Casamino acids, starch, citrate and yeast extract in the presence of oxygen (4 %) and S0 . Growth occurs only at NaCl concentrations below 0.4 % (w/v). The G+C content of strain CR11T is 40.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence places the strain as a close relative of Thermocrinis ruber OC 1/4T (95.7 % sequence similarity). Based on phylogenetic and physiological characteristics, we propose the name Thermocrinis minervae sp. nov., with CR11T (5DSM 19557T 5ATCC BAA-1533T ) as the type strain.
DOI
10.1099/ijs.0.010496-0
Persistent Identifier
http://archives.pdx.edu/ds/psu/10655
Citation Details
Caldwell, Sara L.; Liu, Yitai; Ferrera, Isabel; Beveridge, Terry; and Reysenbach, Anna-Louise, "Thermocrinis minervae sp. nov., A Hydrogen and Sulfur-oxidizing, Thermophilic Member of the Aquificales from a Costa Rican Terrestrial Hot Spring" (2010). Biology Faculty Publications and Presentations. 52.
http://archives.pdx.edu/ds/psu/10655
Description
This is an author manuscript that has been accepted for publication in International Journal of Systematic and Evolutionary Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in International Journal of Systematic and Evolutionary Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission. Copyright 2010 International Union of Microbiological Societies. The Society for General Microbiology and the International Union of Microbiological Societies disclaim any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties.
The final copy-edited, published article, which is the version of record, can be found at http://ijs.sgmjournals.org, and is freely available without a subscription 24 months after publication.