This research was supported by National Science Foundation grant OISE-0968421 and the National Natural Science Foundation of China grant 40972211 and National Science Foundation DEB-1134877 Funding to Reysenbach. BH acknowledges the generous support of Greg Fullmer through the UNLV Foundation.
Frontiers in Microbiology
Geothermal resources -- China, Thermophilic microorganisms -- China -- Tengchong, Thermophilic bacteria -- Isolation, Thermophilic bacteria -- Phylogeny
The order Aquificales (phylum Aquificae) consists of thermophilic and hyperthermophilic bacteria that are prominent in many geothermal systems, including those in Tengchong, Yunnan Province, China. However, Aquificales have not previously been isolated from Tengchong. We isolated five strains of Aquificales from diverse springs (temperature 45.2–83.3°C and pH 2.6–9.1) in the Rehai Geothermal Field from sites in which Aquificales were abundant. Phylogenetic analysis showed that four of the strains belong to the genera Hydrogenobacter, Hydrogenobaculum, andSulfurihydrogenibium, including strains distant enough to likely justify new species ofHydrogenobacter and Hydrogenobaculum. The additional strain may represent a new genus in theHydrogenothermaceae. All strains were capable of aerobic respiration under microaerophilic conditions; however, they had variable capacity for chemolithotrophic oxidation of hydrogen and sulfur compounds and nitrate reduction.
Hedlund, B. P., Reysenbach, A. L., Huang, L., Ong, J. C., Liu, Z., Dodsworth, J. A., ... & Dong, H. (2015). Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China. Frontiers in microbiology, 6.