We gratefully acknowledge the financial support provided by the National Institutes of Health (CA127622 to B.S.M. and AI51629 to K.A.R.) and the Life Sciences Research Foundation via a Tularik postdoctoral fellowship to A.S.E.
Antineoplastic agents, Marine bacteria, Polyketides -- Synthesis, Polyketides -- Metabolism
A new series of coenzyme A-tethered polyketide synthase extender units were discovered in relation to the biosynthesis of the salinosporamide family of anticancer agents from the marine bacterium Salinispora tropica. In vivo and in vitro experiments revealed that the crotonyl-CoA reductase/carboxylase SalG has broad substrate tolerance toward 2-alkenyl-CoAs that give rise to the salinosporamide C-2 substitution pattern.
Liu, Yuan; Hazzard, Christopher; Eustáquio, Alessandra S.; Reynolds, Kevin A.; and Moore, Bradley S., "Biosynthesis of Salinosporamides from α,β-Unsaturated Fatty Acids: Implications for Extending Polyketide Synthase Diversity" (2010). Chemistry Faculty Publications and Presentations. 169.