National Natural Science Foundation of China (Nos. 21472118 and 21672131), the Program for the Top Young and Middle-aged Innovative Talents of Higher Learning Institutions of Shanxi (No. 2013802), Talents Support Program of Shanxi Province (No. 2014401), Shanxi Province Outstanding Youth Fund (No. 2014021002), Shanxi University funds for study abroad 2014, and the National Institutes of Health (No. R15EB016870).
cysteine, Homocysteine, Fluorescent probes -- Design and construction, Thiols, Fluorescent probes -- Effect of pH on
The very close structural similarities between cysteine and homocysteine present a great challenge to achieve their selective detection using regular fluorescent probes, limiting the biological and pathological studies of these two amino thiols. A coumarin-based fluorescent probe was designed featuring pH-promoted distinct turn-on followed by ratiometric fluorescence responses for Cys and turn-on fluorescence response for Hcy through two different reaction paths. These specific responses demonstrate the activity differences between Cys and Hcy qualitatively for the first time. The probe could also be used for Cys and Hcy imaging in living cells.
Yue, Y., Huo, F., Li, X., Wen, Y., Yi, T., Salamanca, J., ... & Yin, C. (2016). pH-Dependent fluorescent probe that can be tuned for cysteine or homocysteine. Organic letters, 19(1), 82-85.