Spontaneous Covalent Self-Assembly of the Azoarcus Ribozyme from Five Fragments
Spontaneous covalent assembly of short RNA fragments has been proposed as a plausible prebiotically relevant pathway to a self‐reproducing system. We previously showed that the Azoarcus group I intron could self‐assemble from four RNA fragments. Here, we extended this fragmentation to five RNAs that averaged <40 nucleotides in length. We optimized this reaction and showed that a dehydration–rehydration sequence was the most effective means to date to shift the self‐assembly equilibrium from reactants to products.
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Jayathilaka, T.S. & Lehman, N. 2017. Spontaneous Covalent Self‐Assembly of the Azoarcus Ribozyme from Five Fragments. ChemBioChem, 19(3):217-220.