Detecting Intracellular ClO- with Ratiometric Fluorescent Signal and its Application in Civo
We thank the National Natural Science Foundation of China (No. 21672131, 21775096, 21705102), Talents Support Program of Shanxi Province (2014401), Shanxi Province Foundation for Returness (2017-026), Shanxi Province Science Foundation for Youths (201701D221061) and Scientific Instrument Center of Shanxi University, Institute of Pharmacology and Toxicology Academy of Military Medical Sciences PLA, Peop. Rep. China.
Sensors and Actuators B: Chemical
BODIPY and coumarin were assembled into a single scaffold that was cleaved upon oxidation by ClO-, thereby enabling ratiometric fluorescent detection ClOâˆ’. The ratiometric fluorescence response was distinctive (|510/|660=18.3) in the presence of ClO-. It was accompanied by an obvious solution color change from dark-blue to colorless. The probe is highly selective and sensitive towards ClO-, with a rapidly response time. Moreover, imaging experiments in live HeLa cells showed that the probe could readily penetrate the cell membrane and was amenable to real-time imaging of ex/endogenous ClO-. Importanty, the probe was successfully used to detect ClO- in vivo via imaging an arthritis nude mouse model.
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Kang, J., Huo, F., Zhang, Y., Chao, J., Strongin, R., Yin, C. (2018). Detecting intracellular ClO- with ratiometric fluorescent signal and its application in vivo, Sensors and Actuators B: Chemical, Volume 273, Pages 1532-1538.