This work was supported in part by grant No. R44GM064299 from the Small Business Innovation Research program of the National Institutes of Health.
DNA microarrays -- Research, Nucleic acid hybridization, Chromosome polymorphism
Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.
Fish, Daniel J., M. Todd Horne, Robert P. Searles, Greg P. Brewood, and Albert S. Benight. "Multiplex SNP discrimination." Biophysical journal 92, no. 10 (2007): L89-L91.