This work was supported by the National Institutes of Health (RO1 EB002044)
Fluorescence spectroscopy, Sulfur amino acids, Molecular probes
A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
Yixing, G., Xiaofeng, Y., Hakuna, L., Barve, A., Escobedo, J. O., Lowry, M., & Strongin, R. M. (2012). A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma. Sensors (14248220), 12(5), 5940-5950. doi:10.3390/s120505940