Published In
Journal of the Optical Society of America A: Optics, Image Science and Vision
Document Type
Article
Publication Date
1-1-2011
Subjects
Microscopy -- Technique -- Innovations, Image processing -- Digital techniques, Microscopes -- Calibration
Abstract
We present a method of using an unmodified differential interference contrast microscope to acquire quantitative information on scatter and absorption of thin tissue samples. A simple calibration process is discussed that uses a standard optical wedge. Subsequently, we present a phase-stepping procedure for acquiring phase gradient information exclusive of absorption effects. The procedure results in two-dimensional maps of the local angular (polar and azimuthal) ray deviation. We demonstrate the calibration process, discuss details of the phase-stepping algorithm, and present representative results for a porcine skin sample.
DOI
10.1364/JOSAA.28.001297
Persistent Identifier
http://archives.pdx.edu/ds/psu/7149
Citation Details
Duncan, D. D., Fischer, D. G., Dayton, A., & Prahl, S. A. (2011). Quantitative Carre differential interference contrast microscopy to assess phase and amplitude. [Article]. Journal of the Optical Society of America a-Optics Image Science and Vision, 28(6), 1297-1306.
Description
This is the publisher's final pdf. This paper was published in Journal of the Optical Society of America A and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: [http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-28-6-1297]. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.