Date of Award
Bachelor of Science (B.S.) in Biology and University Honors
Contrast media (Diagnostic imaging), Nuclear magnetic resonance spectroscopy, Chelates -- Spectra, Magnetic resonance imaging
Current contrast agents are typically been Gd3+-based, which successfully enhance MRI images but are limited in detecting certain analytes compared to new agents that operate through a CEST mechanism. In this study, a Eu3+ and Tm3+ chelate were examined as CEST agents for MRI imaging in four concentrations of zinc: 0, 0.5, 1, and 2 equivalents. T1 values were obtained using NMR spectrometry, which were then used to fit the data to obtain CEST spectra for the compounds. The CEST spectra of the Tm3+ chelate had a shift in the amide protons as Zn2+ concentrations increased indicating a presence of multiple species and a change in coordination chemistry. The change of the amide peak at -52 ppm showed its potential to be used as a marker for Zn2+ concentration. The Eu3+ chelate was found have a distinct change in the water peak shape, intensity and shift as Zn2+ was added, showing increased water exchange kinetics.
King, Diana, "The Effect of Zn2+ Binding on the Chemistry of Tm3+ and Eu3+ Chelates" (2018). University Honors Theses. Paper 516.