Date of Award

3-1-2018

Document Type

Thesis

Degree Name

Bachelor of Science (B.S.) in Biology and University Honors

Department

Biology

First Advisor

Mark Woods

Subjects

Contrast media (Diagnostic imaging), Nuclear magnetic resonance spectroscopy, Chelates -- Spectra, Magnetic resonance imaging

DOI

10.15760/honors.521

Abstract

Current contrast agents are typically been Gd3+-based, which successfully enhance MRI images but are limited in detecting certain analytes compared to new agents that operate through a CEST mechanism. In this study, a Eu3+ and Tm3+ chelate were examined as CEST agents for MRI imaging in four concentrations of zinc: 0, 0.5, 1, and 2 equivalents. T1 values were obtained using NMR spectrometry, which were then used to fit the data to obtain CEST spectra for the compounds. The CEST spectra of the Tm3+ chelate had a shift in the amide protons as Zn2+ concentrations increased indicating a presence of multiple species and a change in coordination chemistry. The change of the amide peak at -52 ppm showed its potential to be used as a marker for Zn2+ concentration. The Eu3+ chelate was found have a distinct change in the water peak shape, intensity and shift as Zn2+ was added, showing increased water exchange kinetics.

Persistent Identifier

http://archives.pdx.edu/ds/psu/24260

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