Date of Award
Bachelor of Science (B.S.) in Biology and University Honors
Archaebacteria -- Genetic aspects, Molecular biology, Thermophilic microorganisms, Reporter genes, Glucosidases, Gene expression
Archaea are the domain of life closest to the Eukaryotes (which include humans), and they share a very similar gene replication system. Pyrococcus furiosus, a thermophilic archaeon, can be used as a model organism to study this replication system. A common tool used to conduct these studies is a reporter gene. In this study, the beta glucosidase gene was attempted to be integrated by a plasmid vector designed to enhance gene activity and assessed for viability for use as a reporter gene in P. furiosus. This tool would be helpful to track levels of gene expression. Though it was not tested in P. furiosus, creating the plasmid gave valuable information on how to optimize the beta glucosidase reporter gene system in P. furiosus.
Carrasco, Arman, "Expressing the β-glucosidase gene as a reporter in the hyperthermophilic archaeon Pyrococcus furiosus" (2018). University Honors Theses. Paper 584.