Date of Award
Bachelor of Science (B.S.) in Health Studies: Health Sciences and University Honors
Archaebacteria -- Genetic aspects, Thermophilic microorganisms, Green fluorescent protein, Gene expression, Plasmids -- Genetics
Archaea are the most recently discovered domain of life, are extremely diverse and can be manipulated for research and commercial use. Pyrococcus furiosus is a hyperthermophilic archaeon used as a model organism for studying archaea, especially extremophiles. Green fluorescent proteins are a commonly used marker of transcription but can only be expressed in a narrow temperature zone and denature at the extreme optimal growth temperature of P. furiosus. To mediate this inability to use green fluorescent protein in hyperthermophiles, a shuttle vector for P. furiosus and Escherichia coli was designed and genes for thermostable green fluorescent proteins were incorporated. This plasmid can be used to upregulate expression of this highly thermostable green fluorescent protein and demonstrate successful transformation. Though the plasmid was not transformed into P. furiosus, the construction of the plasmid and transformation of E. coli were important steps that gave insight into methods of making this transformation and expression possible.
Hollar, Emma R., "Transformation of Pyrococcus furiosus with Thermostable Green Fluorescent Protein" (2018). University Honors Theses. Paper 609.