Drake C. Mitchell
Date of Award
Bachelor of Science (B.S.) in Physics: Biomedical Physics and University Honors
Nanoparticles, Bilayer lipid membranes, Fluorescence spectroscopy, Biomedical engineering
Citrate-coated gold nanoparticles (AuNPs) are increasingly implemented in many biomedical applications due to their novel electrochemical properties and further stabilization against influence from the cellular environment by conjugation of citrate ligands to the exterior of the gold nanocrystalline lattice. AuNPs have the ability to permeate the cell membrane without disturbing the nucleus, with some size regimes, without producing any known toxic effects. This work presents a collection and analysis of time-resolved fluorescence spectroscopy data describing the interior of the bilayer of unsaturated 16:0 - 18:1 phosphatidylcholine large unilamellar vesicles when exposed to 5, 10, and 20 [nm]-diameter citrate-capped AuNPs at physiological temperature. A description of how the membrane was affected at the molecular level was formulated by analyzing frequency-domain lifetime and time-resolved fluorescence anisotropy decay measurements of AuNP-LUV assemblies.
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Vallens, Marie, "Interactions of Phosphatidylcholine Vesicles and Citrate-stabilized Gold Nanoparticles" (2019). University Honors Theses. Paper 692.