Date of Award

5-24-2019

Document Type

Thesis

Degree Name

Bachelor of Science (B.S.) in Physics: Biomedical Physics and University Honors

Department

Physics

First Advisor

Drake C. Mitchell

Subjects

Gold nanoparticles, Lipid membranes -- Biotechnology, Phosphatidylcholine, Fluorescence spectroscopy, Bilayer lipid membranes -- Molecular aspects

DOI

10.15760/honors.710

Abstract

Citrate-coated gold nanoparticles (AuNPs) are increasingly implemented in many biomedical applications due to their novel electrochemical properties and further stabilization against influence from the cellular environment by conjugation of citrate ligands to the exterior of the gold nanocrystalline lattice. AuNPs have the ability to permeate the cell membrane without disturbing the nucleus, with some size regimes, without producing any known toxic effects. This work presents a collection and analysis of time-resolved fluorescence spectroscopy data describing the interior of the bilayer of unsaturated 16:0 – 18:1 phosphatidylcholine large unilamellar vesicles when exposed to 5, 10, and 20 [nm]-diameter citrate-capped AuNPs at physiological temperature. A description of how the membrane was affected at the molecular level was formulated by analyzing frequency-domain lifetime and time-resolved fluorescence anisotropy decay measurements of AuNP-LUV assemblies.

Persistent Identifier

https://archives.pdx.edu/ds/psu/28806

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