Date of Award

5-31-2019

Document Type

Thesis

Degree Name

Bachelor of Arts (B.A.) in Biology and University Honors

Department

Biology

First Advisor

Bradley Buckley

Subjects

Mytilus edulis -- Oregon -- Pacific Coast, Mytilus edulis -- Effect of water temperature on, Mytilus edulis -- Efffect of salinity on, Body temperature -- Regulation, Ocean temperature -- Oregon -- Pacific Coast

DOI

10.15760/honors.747

Abstract

Marine life is highly susceptible to stress and physiological impact through climate change, as this directly alters thermal temperatures and salinity concentrations of the ocean. The coastal species, Mytilus edulis, a mussel found variously in the northern hemisphere, was selected for cellular stress response testing. Through the investigation of temperature and salinity effects on Mytilus edulis using separation group exposure and flow cytometry analysis, it was found that salinity changes had significantly impacted the cellular division process. Individuals who received the environmentally controlled salinity concentration of 32ppt had abundant cells staged in the S phase of cellular division. Mussels exposed to salinity concentrations of 16ppt had reduced cells in the S phase, with the highest peak shifted to the left, indicative of increased cells in G0/G1 phase. In comparing the groups exposed to 13˚C versus 21˚C, similar results were seen, with the higher temperature being the advantaged group. Future testing using several varying temperatures between ranges of 5˚C to 30˚C may provide more information on how ocean warming can directly impact cellular response of Mytilus edulis.

Persistent Identifier

https://archives.pdx.edu/ds/psu/28917

Share

COinS