Advisor

John Perona

Date of Award

Summer 10-2-2014

Document Type

Thesis

Degree Name

Master of Science (M.S.) in Chemistry

Department

Chemistry

Physical Description

1 online resource (vi, 74 pages)

Subjects

Aminoacyl-tRNA synthetases, RNA-protein interactions

DOI

10.15760/etd.2005

Abstract

Several Saccharomyces cerevisiae mutant tRNAQ2 species (glutamine isoacceptor, CUG anticodon) were synthesized and assayed for aminoacylation activity with Saccharomyces cerevisiae glutaminyl-tRNA synthetase. The derived steady state parameters were compared to similar datasets from the literature. The mutants behaved analogously to similar mutant species based on tRNA from Escherichia coli, but with slightly relaxed specificity as revealed by comparison of kcat/KM values relative to wild type in vitro transcribed tRNA. Additionally the eukaryotic N-terminal domain appendage, as found in Sce glutaminyl-tRNA synthetase, is considered in light of the discovery of non-canonical aminoacyl-tRNA synthetase functions, including its role in the assembly of the multiple aminoacyl-tRNA synthetase complex.

Description

Supplemental Data (SD) file descriptions:

SD1 (143 KB) - FASTA sequences of Eukaryotic and Bacterial QRS sequences used for Figure S1; can be opened with text editor

SD2 (15 KB) - FASTA sequences of Eco tRNA; can be opened with text editor SD3 (51 KB) - FASTA sequences of Sce tRNA; can be opened with text editor

SD4 (99 KB) - FASTA sequences of Hsa tRNA; can be opened with text editor

SD5 (289,388 KB) - Zip file of raw kinetic data (.gel); requires ImageQuant to open

SD6 (68 KB) - Spreadsheet (.xml) legend to eukaryotic QRS sequences in SD1; requires a spreadsheet application to open

Persistent Identifier

http://archives.pdx.edu/ds/psu/12745

303099_supp_C546853A-120A-11E4-BDE6-2D632E1BA5B1.fasta (142 kB)
FASTA sequences of Eukaryotic and Bacterial QRS sequences used for Figure S1

303099_supp_E04D2FAA-120A-11E4-B40B-C642EF8616FA.fasta (50 kB)
FASTA sequences of Sce tRNA

303099_supp_D2759D86-120A-11E4-88A2-4A632E1BA5B1.fasta (14 kB)
FASTA sequences of Eco tRNA

303099_supp_EB592AD4-120A-11E4-9A75-0C642E1BA5B1.fasta (98 kB)
FASTA sequences of Hsa tRNA

303099_supp_87A8E258-120B-11E4-8A45-6A43EF8616FA.zip (289387 kB)
Zip file of raw kinetic data

303099_supp_9C9F4C74-120B-11E4-9922-3945EF8616FA.xml (67 kB)
Spreadsheet (.xml) legend to eukaryotic QRS sequences in SD1

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Chemistry Commons

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