Advisor

Robert M. Strongin

Date of Award

1-1-2012

Document Type

Dissertation

Degree Name

Doctor of Philosophy (Ph.D.) in Chemistry

Department

Chemistry

Physical Description

1 online resource (xi, 88 pages)

Subjects

Chemosensor, Fluorescent indicators, Disease biomarkers, Adenolosuccinate lyase (ADSL), Excitation-emission matrix, Thiols, Fluorescent probes, Biochemical markers -- Diagnostic use, Optical detectors

DOI

10.15760/etd.262

Abstract

Xanthene dyes are common fluorophores which have been widely used as molecular probes. The xanthene fluorophores can be used as highly selective optical sensors to detect disease biomarkers. A new fluorogenic dye containing an alpha, beta-unsaturated aldehyde moiety exhibits selective fluorescent signal enhancement in the presence of cysteine or peptides containing N-terminal cysteine residues. The mechanism is based on synergistic covalent and supramolecular interactions. A unique rhodamine boronic acid indicator is used in an optimized data collection protocol for wavelength- and time-dependent selectivity of various saccharides and nucleosides. One indicator is thereby capable of selectively distinguishing structurally related analytes in mixtures. Moreover, the rhodamine-based boronic acid responds linearly to increasing riboside concentrations in urine samples, potentially enabling the screening for inborn purine metabolism disorders.

Description

Portland State University. Dept. of Chemistry

Persistent Identifier

http://archives.pdx.edu/ds/psu/7243

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