Portland State University. Department of Chemistry
Robert M. Strongin
Date of Award
Doctor of Philosophy (Ph.D.) in Chemistry
1 online resource (xi, 88 pages)
Chemosensor, Fluorescent indicators, Disease biomarkers, Adenolosuccinate lyase (ADSL), Excitation-emission matrix, Thiols, Fluorescent probes, Biochemical markers -- Diagnostic use, Optical detectors
Xanthene dyes are common fluorophores which have been widely used as molecular probes. The xanthene fluorophores can be used as highly selective optical sensors to detect disease biomarkers. A new fluorogenic dye containing an alpha, beta-unsaturated aldehyde moiety exhibits selective fluorescent signal enhancement in the presence of cysteine or peptides containing N-terminal cysteine residues. The mechanism is based on synergistic covalent and supramolecular interactions. A unique rhodamine boronic acid indicator is used in an optimized data collection protocol for wavelength- and time-dependent selectivity of various saccharides and nucleosides. One indicator is thereby capable of selectively distinguishing structurally related analytes in mixtures. Moreover, the rhodamine-based boronic acid responds linearly to increasing riboside concentrations in urine samples, potentially enabling the screening for inborn purine metabolism disorders.
Lim, Soojin, "Fluorescent Indicators for Disease Biomarkers" (2012). Dissertations and Theses. Paper 262.