Portland State University. Department of Chemistry
Joann S. Loehr
Date of Publication
Master of Science (M.S.) in Chemistry
Glutamic acid, Ligands, Hemoerythrin
1 online resource (46 p.)
The amino acid sequence determination of Phascolopsis gouldii hemerythrin in the region of the proposed iron ligand at position 58 was the main objective of this research endeavor. Generation of a large peptide was pursued by trypsin digestion of citraconylated hemerythrin producing peptide 50-113 for sequenator analysis. Detection of the phenylthiohydantoin amino acid derivatives by gas-liquid and high-performance-liquid chromatography yielded unambiguous sequence elucidation through the region of interest identifying residue 58 as glutamic acid.
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Gormley, Patricia M., "New evidence supporting the assignment of glutamic acid as an iron ligand in hemerythrin" (1978). Dissertations and Theses. Paper 2783.