Portland State University. Department of Biology
Mary L. Taylor
Date of Award
Master of Science (M.S.) in Biology
1 online resource (53 p.)
Plasmids, Arsenic, R factors, Escherichia coli
The resistance transfer factor R45 from Escherichia coli confers inducible arsenate and arsenite resistance in that bacterium. The genes for these resistances were cloned into the EcoRl - Sphl multiple cloning site of PGEM3 Blue vector (Promega) to produce a 4.9 kilobase plasmid, pJC1. This recombinant plasmid, pJC1, conferred IPTG induced resistance to arsenite and arsenate. In addition, pJCl was tested for homology with the E. coli plasmid R773, which encodes for arsenic resistance in that bacterium as well.
Through DNA-DNA hybridization the arsenic resistance determinants of R45 and R773 were compared. Under stringent hybridization conditions, R45 demonstrated DNA sequence homology to the ArsB and Ars C genes of R773 but not to the ArsA gene of R773.
Clark, Joshua T., "Determination of homology between the arsenic resistance plasmids R45 and R773 in Escherichia coli" (1988). Dissertations and Theses. Paper 3759.