Synthesis, chemistry, fluorophore, nerve, reagent, chromatography, oxazine, scaffold
Nerve tissue damage as a result of surgical injury is a common negative outcome during many surgical interventions. Depending on the site of the injury and the amount of cellular damage, nerve tissue injury may potentially have a life altering effect on the surgical patient. Post-surgical nerve pain is often a chronic problem which persists long after injuries have healed, and can severely infringe upon quality of life for individuals.
Surgery that is fluorescence guided will aid in the visualization of nerve structure patterns, and as a result may significantly improve patient outcomes. Small molecule fluorophores may potentially cross the blood brain barrier and blood nerve barrier, allowing unparalleled future application in surgical interventions involving the central and peripheral nervous systems. In this research, work was done to synthesize a small set of fluorescent contrast agents which may have intraoperative application to highlight nerve tissue in real time, with great sensitivity and in high contrast against background tissues. Though work is ongoing, absorption and fluorescence spectra will be observed as a way to evaluate each synthesized agent. Systemic administration of the novel agents in rodents will help define nerve tissue uptake specificity. In order to evaluate nerve signal to background ratios, images of surgical sites will be collected and quantified. The knowledge garnered from this study will greatly help inform nerve tissue specific, clinical grade, contrast agent development.
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Mathieson, Cassandra D.
"Nerve Tissue Targeted Fluorophore Synthesis: From Scheme to Administration,"
PSU McNair Scholars Online Journal:
1, Article 7.