The authors would like to gratefully acknowledge the support of the National Science Foundation through Award No. DBI-0500812 for Biological Sciences Instrument Development. Tip fabrication was assisted through the NSF support (ECCS-NSF-0520891, with additional support from PSU, ONAMI/ONR Award Nos. (#N00014-07-1-0457), (#N00014-08-1-1237), and (#N00014-10-1-0082)), and additional personnel support for Zechariah Dzegede and Cliff Kim under the NSF DMR REU site Grant No. (NSF-069280).
Review of Scientific Instruments
Field programmable gate arrays, Fluorescence spectroscopy, Atmospheric pressure
The increasing popularity of nanometrology and nanospectroscopy has pushed researchers to develop complex new analytical systems. This paper describes the development of a platform on which to build a microscopy tool that will allow for flexibility of customization to suit research needs. The novelty of the described system lies in its versatility of capabilities. So far, one version of this microscope has allowed for successful near-field and far-field fluorescence imaging with single molecule detection sensitivity. This system is easily adapted for reflection, polarization (Kerr magneto-optical (MO)), Raman, super-resolution techniques, and other novel scanning probe imaging and spectroscopic designs. While collecting a variety of forms of optical images, the system can simultaneously monitor topographic information of a sample with an integrated tuning fork based shear force system. The instrument has the ability to image at room temperature and atmospheric pressure or under liquid. The core of the design is a field programmable gate array (FPGA) data acquisition card and a single, low cost computer to control the microscope with analog control circuitry using off-the-shelf available components. A detailed description of electronics, mechanical requirements, and software algorithms as well as examples of some different forms of the microscope developed so far are discussed.
Nowak, D. B., Lawrence, A. J., Dzegede, Z. K., Hiester, J. C., Kim, C., & Sánchez, E. J. (2011). Field programmable gate array based reconfigurable scanning probe/optical microscope. Review Of Scientific Instruments, 82(10), 103701.