Published In

Journal of Vacuum Science and Technology A

Document Type

Article

Publication Date

2-12-2015

Subjects

Immoblized ligands (Biochemistry), Activation (Chemistry)

Abstract

Perfluorophenylazide (PFPA) chemistry is a novel method for tailoring the surface properties of solid surfaces and nanoparticles. It is general and versatile, and has proven to be an efficient way to immobilize graphene, proteins, carbohydrates, and synthetic polymers. The main thrust of this work is to provide a detailed investigation on the chemical composition and surface density of the PFPA tailored surface. Specifically, gold surfaces were treated with PFPA-derivatized (11-mercaptoundecyl) tetra(ethylene glycol) (PFPA-MUTEG) mixed with 2-[2-(2-mercaptoethoxy)ethoxy]ethanol (MDEG) at varying solution mole ratios. Complementary analytical techniques were employed to characterize the resulting films including Fourier transform infrared spectroscopy to detect fingerprints of the PFPA group, x-ray photoelectron spectroscopy and ellipsometry to study the homogeneity and uniformity of the films, and near edge x-ray absorption fine structures to study the electronic and chemical structure of the PFPA groups. Results from these studies show that the films prepared from 90:10 and 80:20 PFPA-MUTEG/MDEG mixed solutions exhibited the highest surface density of PFPA and the most homogeneous coverage on the surface. A functional assay using surface plasmon resonance with carbohydrates covalently immobilized onto the PFPAmodified surfaces showed the highest binding affinity for lectin on the PFPA-MUTEG/MDEG film prepared from a 90:10 solution.

Description

Copyright 2015 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.

The following article appeared in Zorn, G., Castner, D. G., Tyagi, A., Wang, X., Wang, H., & Yan, M. (2015). Analysis of the surface density and reactivity of perfluorophenylazide and the impact on ligand immobilization. Journal of Vacuum Science & Technology A,33(2), 021407. and may be found at http://dx.doi.org/10.1116/1.4907924

Present address for Mingdi Yan, University of Massachusetts at Lowell.

DOI

10.1116/1.4907924

Persistent Identifier

http://archives.pdx.edu/ds/psu/14444

Included in

Chemistry Commons

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