Selective Fluorescence Detection of Cysteine and N-Terminal Cysteine Peptide Residues

Authors

Soojin Lim, Portland State University
Jorge O. Escobedo, Portland State UniversityFollow
Mark Lowry, Portland State UniversityFollow
Xiangyang Xu, Portland State University
Robert M. Strongin, Portland State UniversityFollow

Published In

Support from the National Institutes of Health via award RO1 EB002044 is gratefully acknowledged

Document Type

Post-Print

Publication Date

2010

Subjects

Supramolecular chemistry, Cysteine -- Detection, Fluorescent probes

Abstract

A new fluorogenic fluorescein derivative containing an α,β-unsaturated aldehyde moiety produces a selective fluorescent signal enhancement in the presence of cysteine or peptides containing N-terminal cysteine residues. The mechanism is based on synergistic covalent and supramolecular interactions.

Description

This is the author’s version of a work that was accepted for publication in Chemical Communications (Cambridge, England). Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemical Communications (Cambridge, England), 2010, 46(31):5707-9. doi: 10.1039/c0cc01398f.

Reproduced by permission of The Royal Society of Chemistry (RSC)

DOI

10.1039/c0cc01398f

Persistent Identifier

http://archives.pdx.edu/ds/psu/11117

Citation Details

Lim, Soojin; Escobedo, Jorge O.; Lowry, Mark; Xu, Xiangyang; and Strongin, Robert M., "Selective Fluorescence Detection of Cysteine and N-Terminal Cysteine Peptide Residues" (2010). Chemistry Faculty Publications and Presentations. 54.
http://archives.pdx.edu/ds/psu/11117