Date of Award

2014

Document Type

Thesis

Department

Biology

First Advisor

Jeffrey Singer

Subjects

Ubiquitin, Protein binding

DOI

10.15760/honors.48

Abstract

Ubiquitination is a fundamental biochemical process, which controls numerous aspects of protein function, such as degradation, protein-protein interaction and sub cellular localization. Cullin 3 (Cul3) is a ubiquitin ligase that is fundamentally responsible for ubiquitination of proteins and plays a critical role in normal cellular processes. Cul3 has been shown to use substrate adaptors to facilitate the ubiquitination process and many of these substrate adaptors contain BTB domains among other protein-protein interacting domains. A novel BTB domain containing protein is Ctb73. Ctb73 has a BACK and Kelch domain along with a BTB domain. It has not been shown whether Ctb73 is a substrate adaptor for Cul3. The purpose of this experiment is to determine whether Ctb73 binds to Cul3 and if so, through which domain.

Comments

An undergraduate honors thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in University Honors and Micro/Molecular Biology

Persistent Identifier

http://archives.pdx.edu/ds/psu/12466

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