First Advisor
Samantha Underwood
Date of Award
Spring 6-2024
Document Type
Thesis
Degree Name
Bachelor of Science (B.S.) in Biology and University Honors
Department
Biology
Language
English
Subjects
TBI, Traumatic Brain Injuries, ICH, intracranial hemorrhage, ELISA, S100B, GFAP
Abstract
Background: Traumatic Brain Injuries (TBI) and Intracranial Hemorrhages (ICH) are one of the leading causes of death. Faster diagnosis and treatment positively impact patient outcomes. Two protein markers (UCHL-1 and GFAP) were approved by the FDA as an indicator for prediction indicators of an ICH or moderate TBI.
Methods: In this project, the S100 Calcium Binding Protein B (S100B) was measured by performing an enzyme-linked immunoglobulin assay (ELISA) on the placebo arm of the Prehospital Tranexamic Acid Use for Traumatic Brain Injury" clinical trial. The aim of this project is to compare glial fibrillary acidic protein (GFAP) concentrations that were previously collected by Anderson et al. to compare the reliability of both proteins as protein markers for ICH (2020). Since this project is still in the preliminary stages, it focuses on testing the effectiveness and reliability of three different ELISA kits (LS Bio, My Bio Source, and Millipore) available on the market.
Results: Millipore, an ELISA kit that had the largest detection range (2.0 - 2000 pg./mL) was found to be the most effective kit to utilize. LS Bio and My Bio Source had the same detection range (31.25 - 2000 pg./mL), but most of the S100B concentrations were not within the detection range.
Conclusion: When utilizing kits to test out biomarkers, it is important to use reliable kits to get the most reliable and accurate data. Accurate concentrations allow for further understanding of potential biomarkers for diseases and injuries of patients.
Recommended Citation
Phan, Anthony, "A Thorough Examination of Different ELISA Kits to Determine the Concentration of S100B linked to Intracranial Hemorrhage" (2024). University Honors Theses. Paper 1551.