First Advisor

Elaine Spencer

Term of Graduation

Spring 1969

Date of Publication

5-29-1969

Document Type

Thesis

Degree Name

Master of Arts (M.A.) in Chemistry

Department

Chemistry

Language

English

Subjects

Dehydrogenases

DOI

10.15760/etd.458

Physical Description

1 online resource (vi, 76 pages)

Abstract

Very little is known about the effect of hydrofluoric acid and of the fluoride ion on enzyme systems. The purpose of this work was to determine the effect of hydrofluoric acid and of the fluoride ion on the enzyme, yeast alcohol dehydrogenase and to distinguish between the effect of the fluoride ion and of hydrofluoric acid. The rate of the enzyme reaction was followed spectrophotometrically at 340 mμ on the Cary 14 Model spectrophotometer according to the method of Racker. The data taken from the instrument recordings were plotted on two types of graphs, the Lineweaver-Burk plot and the Hanes plot. Conclusions were drawn from the calculations made on these plots.

Inhibition studies were run using KCI, NaCl, KF and NaF varying in concentration from 0.001 to 0.12 M at two different pH levels. For the fluoride salts, this gave a concentration of HF which varied from 8.94 x 10ˉ⁸ to 1.07 x 10ˉ⁵ M at pH 7.5 and 8.94 x 10ˉ̄⁹ to 1.07 x 10-⁶ M at pH 8.5

The fluoride salts showed no greater inhibition than the chloride salts at either pH. Since there is no difference in inhibition between the two types of salts, the inhibition cannot be attributed to the presence of hydrofluoric acid. If the inhibition had been due to hydrofluoric acid, we would have observed a greater inhibition with the fluoride salts than with the chloride salts since hydrochloric acid is 100% ionized.

Rights

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

Comments

If you are the rightful copyright holder of this dissertation or thesis and wish to have it removed from the Open Access Collection, please submit a request to pdxscholar@pdx.edu and include clear identification of the work, preferably with URL.

Persistent Identifier

https://archives.pdx.edu/ds/psu/9204

Included in

Chemistry Commons

Share

COinS