Genome sequencing -- Techniques -- Evaluation, Spiders -- Genome mapping -- Analysis, Phylogenetics -- Effect of technological innovations on, Polymerase chain reaction -- Methodology
The development of second generation sequencing technology has resulted in the rapid production of large volumes of sequence data for relatively little cost, thereby substantially increasing the quantity of data available for phylogenetic studies. Despite these technological advances, assembling longer sequences, such as that of entire mitochondrial genomes, has not been straightforward. Existing studies have been limited to using only incomplete or nominally intra-specific datasets resulting in a bottleneck between mitogenome amplification and downstream high-throughput sequencing. Here we assess the effectiveness of a wide range of targeted long-range PCR strategies, encapsulating single and dual fragment primer design approaches to provide full mitogenomic coverage within the Araneae(Spiders).
Briscoe AG, Goodacre S, Masta SE, Taylor MI, Arnedo MA, et al. (2013) Can Long-Range PCR Be Used to Amplify Genetically Divergent Mitochondrial Genomes for Comparative Phylogenetics? A Case Study within Spiders (Arthropoda: Araneae). PLoS ONE 8(5): e62404.