A New Symbiotic Nanoarchaeote (Candidatus Nanoclepta Minutus) and its Host (Zestosphaera Tikiterensis gen. nov., sp. nov.) From a New Zealand Hot Spring
National Science Foundation (DEB-1134877), the National Aeronautics and Space Administration (NNX16AJ66G), and the Geothermal Resources of New Zealand (GRN) Program at GNS Science.
Systematic and Applied Microbiology
Three thermophilic Nanoarchaeota-Crenarchaeota symbiotic systems have been described. We obtained another stable anaerobic enrichment culture at 80Â°C, pH 6.0 from a New Zealand hot spring. The nanoarchaeote (Ncl-1) and its host (NZ3T) were isolated in co-culture and their genomes assembled. The small (âˆ¼200nm) flagellated cocci were often attached to larger cocci. Based on 16S rRNA gene similarity (88.4%) and average amino acid identity (52%), Ncl-1 is closely related to Candidatus Nanopusillus acidilobi. Their genomes both encode for archaeal flagella and partial glycolysis and gluconeogenesis pathways, but lack ATP synthase genes. Like Nanoarchaeum equitans, Ncl-1 has a CRISPR-Cas system. Ncl-1 also relies on its crenarchaeotal host for most of its biosynthetic needs. The host NZ3T was isolated and grows on proteinaceous substrates but not on sugars, alcohols, or fatty acids. NZ3T requires thiosulfate and grows best at 82Â°C, pH 6.0. NZ3T is most closely related to the Desulfurococcaceae, Ignisphaera aggregans (âˆ¼92% 16S rRNA gene sequence similarity, 45% AAI). Based on phylogenetic, physiological and genomic data, Ncl-1 and NZ3T represent novel genera in the Nanoarchaeota and the Desulfurococcaceae, respectively, with the proposed names Candidatus Nanoclepta minutus and Zestosphaera tikiterensis gen. nov., sp. nov., type strain NZ3T (=DSMZ 107634T=OCM 1213T).
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John, E. S., Liu, Y., Podar, M., Stott, M. B., Meneghin, J., Chen, Z., ... & Reysenbach, A. L. (2018). A New Symbiotic Nanoarchaeote (Candidatus Nanoclepta minutus) and its Host (Zestosphaera tikiterensis gen. nov., sp. nov.) from a New Zealand Hot Spring. Systematic and applied microbiology.