Prostate Cancer -- Detection
Current methods for non-invasive prostate cancer (PrCa) detection have a high false-positive rate and often result in unnecessary biopsies. Previous work has suggested that urinary volatile organic compound (VOC) biomarkers may be able to distinguish PrCa cases from benign disease. The behavior of the nematode has been proposed as a tool to take advantage of these potential VOC profiles. To test the ability of Bristol N2 to distinguish PrCa cases from controls, we performed chemotaxis assays using human urine samples collected from men screened for PrCa. Behavioral response of nematodes towards diluted urine from PrCa cases were compared to controls with cancer-free controls. Overall, we observed a significant attraction of young adult-stage nematodes to 1:100 diluted urine from confirmed PrCa cases and repulsion of to urine from controls. When chemotaxis index was considered alongside prostate-specific antigen levels for distinguishing cancer from cancer-free controls, the accuracy of patient classification was 81%. We also observed behavioral attraction of to two previously reported VOCs to be increased in PrCa patient urine. We conclude nematode behavior distinguishes PrCa case urine from controls in a dilution-dependent manner.
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Thompson, M., Feria, N. S., Yoshioka, A., Tu, E., Civitci, F., Estes, S., & Wagner, J. T. (2021). A Caenorhabditis elegans Behavioral Assay Distinguishes Early Stage Prostate Cancer Patient Urine from Controls. Biology Open, bio.057398. https://doi.org/10.1242/bio.057398