Enlarging the Stokes Shift by Weakening the π-Conjugation of Cyanines for High Signal-To-Noise Ratiometric Imaging
The authors thank the National Natural Science Foundation of China (No. 21907062, 22074084), Innovative Talents of Higher Education Institutions of Shanxi, Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (2019L0031), Program of the State Key Laboratory of Quantum Optics and Optical Quantum Devices of Shanxi University (KF202108), Shanxi Province “1331 project” key innovation team construction plan cultivation team (2018-CT-1), Key R&D Program of Shanxi Province (201903D421069).
Organic fluorescent dyes -- Research and Analysis
The signal-to-noise ratio (SNR) is one of the key features of a fluorescent probe and one that often defines its potential utility for in vivo labeling and analyte detection applications. Here, it is reported that introducing a pyridine group into traditional cyanine-7 dyes in an asymmetric manner provides a series of tunable NIR fluorescent dyes (Cy-Mu-7) characterized by enhanced Stokes shifts (≈230 nm) compared to the parent cyanine 7 dye (nm). The observed Stokes shift increase is ascribed to symmetry breaking of the Cy-Mu-7 core and a reduction in the extent of conjugation. The fluorescence signals of the Cy-Mu-7 dyes are enhanced upon confinement within the hydrophobic cavity of albumin or via spontaneous encapsulation within micelles in aqueous media. Utilizing the Cy-Mu-7, ultra-fast in vivo kidney labeling in mice is realized, and it is found that the liver injury will aggravate the burden of kidney by monitoring the fluorescence intensity ratio of kidney to liver. In addition, Cy-Mu-7 could be used as efficient chemiluminescence resonance energy transfer acceptor for the reaction between H O and bisoxalate. The potential utility of Cy-Mu-7 is illustrated via direct monitoring fluctuations in endogenous H O levels in a mouse model to mimic emergency room trauma.
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Locate the Document
Yue, Y., Zhao, T., Xu, Z., Chi, W., Chai, X., Ai, J., ... & Yin, C. (2022). Enlarging the Stokes Shift by Weakening the π‐Conjugation of Cyanines for High Signal‐to‐Noise Ratiometric Imaging. Advanced Science, 2205080.