Date of Award
Bachelor of Science (B.S.) in Biology and University Honors
fluorescence guided surgery, cancer, fluorophore, affibody, biomedical engineering
Evaluation of the compatibility of a nerve-specific fluorophore and a tumor-specific affibody molecule for use in the field of fluorescence guided surgery (FGS) to improve tumor resection and decreased incidence of iatrogenic nerve damage. A novel near-infrared fluorophore, LGW05-75, and a fluorescently labeled affibody molecule that is in Phase 0 clinical trials, ABY-029, were used to specifically highlight tumor and nerve, respectively. To improve solubility, LGW05-75 was encapsulated in micelles and the pharmacokinetics were studied herein with and without the presence of a tumor to enable selection of the optimal time point for nerve to tumor contrast. A human head and neck cancer cell line, Detroit 562, which has high expression of epidermal growth factor receptors (EGFR) was used for this study to evaluate tumor-specific targeting using ABY-029. The studies are still ongoing, but initial results look promising with both nerve- and tumor-specificity shown.
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Szafran-Reeder, Dani, "Multicolor Fluorescence Guided Surgery for Head and Neck Cancer" (2022). University Honors Theses. Paper 1239.
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