First Advisor

Angela Ozburn

Date of Award

Summer 8-16-2022

Document Type

Thesis

Degree Name

Bachelor of Science (B.S.) in Biology and University Honors

Department

Biology

Language

English

Subjects

Circadian, alcohol, nucleus accumbens, suprachiasmatic nucleus, ventral tegmental area, gene expression

Abstract

Previous studies 1) support a role of circadian genes in regulating alcohol intake, and 2) reveal that harmful alcohol use alters circadian rhythms. However, there minimal knowledge of the effects of chronic alcohol processes on rhythmic circadian gene expression across brain regions important for circadian biology and alcohol intake. Therefore, the present study sought to test the effects of chronic binge-like drinking on diurnal circadian gene rhythms in the master circadian pacemaker (SCN), the ventral tegmental area (VTA), and the nucleus accumbens (NAc) in High Drinking in the Dark-1 (HDID-1) mice, a unique genetic risk model for drinking to intoxication. Consistent with earlier findings, we found that 8-weeks of binge-like drinking reduced the amplitude of several core circadian clock genes in the NAc and SCN (but not VTA). To better inform the use of circadian-relevant pharmacotherapies in reducing harmful drinking and ameliorating alcohol’s effects on circadian gene expression, we tested whether the casein kinase-1 inhibitor, PF-670462, or the phosphodiesterase type-4 (an upstream regulator of circadian signalling) inhibitor, apremilast, would reduce binge-like intake and mitigate circadian gene suppression. PF-670462 did not reduce intake, but did have circadian gene effects. In contrast, apremilast reduced drinking, but had no effect on circadian expression patterns.

Rights

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

Persistent Identifier

https://archives.pdx.edu/ds/psu/38495

Available for download on Saturday, August 03, 2024

Included in

Biology Commons

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