Date of Award
Bachelor of Science (B.S.) in Chemistry and University Honors
Activity-based probes, Pathogen research, ubiquitin modification, ubiquitin-like modifiers, Sumo signaling
Research on pathogen intervention and ubiquitin modification has increased over the last few years but a lot is still unknown about ubiquitin-like modifiers (Ubl) and how or if they are modified by pathogens in the same way as Ubiquitin. In this work, two different studies conducted over the course of 2 years are highlighted. The first study focused on purifying and synthesizing four activity based probes in order to study enzymatic activity in the Ubl-modification systems. These probes were reacted with virulence factors isolated from different bacteria and it was concluded that there were unexpected bands formed between some of the probes and the bacterial proteins. In the second study, a SUMOreal assay was developed to investigate if bacteria regulate host SUMO signaling. Past pathogen research has shown that pathogens have ways of hijacking ubiquitin systems with their unique ligases, eliminating unwanted cellular proteins, and creating systems where they are able to control the environment for their own benefit. Both these studies helped with better understanding the roles of these modifiers in bacterial infection in order to ultimately understand crucial mechanisms of disease and aid in the elucidation of fundamental cellular biology.
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Guzman Gonzalez, Danna Eloisa; Pruneda, Jonathan N.; and Divine, Michael, "Understanding Ubiquitin-like Signaling at the Host-Pathogen Interface" (2023). University Honors Theses. Paper 1356.
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