Date of Award
Nile tilapia -- Climatic factors, Climatic changes -- Nile River Watershed, Apoptosis, Protein-protein interactions
Nile tilapia provide an ideal model to study the accumulation of climate change-induced stressors due to their habitat in tropical freshwater systems that are susceptible to salt water invasion and temperature fluctuation due to sea level rise. This study investigates the physiological responses of Nile tilapia to salinity- and temperature-induced stress to identify the interactive effects of simultaneous stressors on the cell cycle. Proteins indicative of cell cycle activity and arrest were quantified in the tissues of Nile tilapia exposed to various environmental conditions varying by temperature and salinity. Dot Blotting was utilized to measure Gadd45 while Western Blotting was used to confirm the identity of the protein. Results from previous investigations in the Buckley lab were also utilized to compare the expression patterns of Gadd45 to relevant cell activity proteins p53 and PCNA. Of the three proteins under scrutiny, a significant change in expression of p53 was observed for the interaction of salinity- and temperature- induced stress, while full strength saltwater treatment alone was found to have a significant impact on the expression of p53, Gadd45 and PCNA. These findings can be used in collaboration with existing studies to describe the coordination of apoptotic pathways in Nile tilapia and other non-mammalian species, as well as to create policy on how to conserve this important species effectively both in the wild and in fisheries for aquaculture, commercial and public health initiatives in response to global climate change.
McGuire, Charlee, "Interactive Physiological Responses of Nile Tilapia to Salinity- and Temperature-induced Stress: Quantifying the Regulation of Apoptosis and Cell Cycle Arrest in Oreochromis Niloticus" (2017). University Honors Theses. Paper 469.