First Advisor

Thelma N. Fisher

Term of Graduation

Spring 1977

Date of Publication


Document Type


Degree Name

Master of Science (M.S.) in Biology






Herpes simplex virus, Tissue culture, Fishes



Physical Description

1 online resource (2, 39 pages)


The purpose of this investigation was to observe changes induced by types 1 and 2 herpes simplex viruses (HSV 1 and HSV 2) when these viruses were cultivated in fish cells. The possibility of attempting to use human strains of herpesviruses in order to transform normal fish tissue culture preparations was considered. Three different fish cell cultures were tested: CHSE-214 cell lines were derived from embryonic chinook salmon (Oncorhynchus tshawytscha), and STE-137 cell lines were obtained from embryonic steelhead trout (Salmo gairdneri). Cells isolated from immature ovaries of rainbow trout (Salmo gairdneri) were propagated by the investigator in these studies.

Both HSV 1 and HSV 2 demonstrated cytopathic effects on the 3 different cell types described above when these cells were observed during the exponential growth phase. If virus was added before cells were dividing actively, or when the cells were maintained in a stationary phase of growth, no effect was observed.

Several methods were employed in an attempt to transform fish cells grown in tissue culture. Different quantities of live virus, preparations of ultraviolet light inactivated virus and purified viral DNA were used in these studies but malignant conversion by known viral components was not achieved.

In these experiments, the 2B strain of cells obtained originally from immature ovaries of rainbow trout appeared to undergo spontaneous transformation thereby becoming established as a permanent cell line. To date tissue cultures designated 2B have undergone 25 transfers in this laboratory.


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