Sponsor
Portland State University. Department of Biology
Advisor
Byron E. Lippert
Date of Award
1979
Document Type
Thesis
Degree Name
Master of Science (M.S.) in Biology
Department
Biology
Physical Description
1 online resource (52 p.)
Subjects
Closterium ehrenbergii
DOI
10.15760/etd.2876
Abstract
Methods were devised for the isolation and pure culture in defined mineral medium of Closteriurn ehrenbergii Menegh (Chlorophyceae). The effect of pH, type of nitrogen source, nitrate concentration, total hardness, and various buffers on growth were investigated. Best growth rates were obtained at pH 8 to 9, but good growth was observed even at pH 9.4. Nitrate, ammonia, or urea nitrogen could be utilized, but at concentrations greater than 10-2M urea was toxic to growth. At higher concentrations of ammonia and nitrate longer lag phases of growth were obtained but eventual higher cell yields were obtained. Good growth rates were obtained with Tris-(hydroxymethyl)-aminomethae buffer at up to l0-2M; higher concentrations were inhibitory.
Persistent Identifier
http://archives.pdx.edu/ds/psu/17349
Recommended Citation
Renstrom, David Bruce, "Culture and growth of Closterium ehrenbergii (Desmidiaceae)" (1979). Dissertations and Theses. Paper 2882.
https://pdxscholar.library.pdx.edu/open_access_etds/2882
10.15760/etd.2876
Description
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