Portland State University. Department of Physics
Term of Graduation
Date of Publication
Doctor of Philosophy (Ph.D.) in Applied Physics
Peptide antibiotics, Lipid membranes -- Effect of drugs on, Bilayer lipid membranes
1 online resource (xv, 128 pages)
Antimicrobial peptides (AMPs) are one of the most promising solutions to drug-resistant bacteria. Melittin and magainin 2 are two of the most representative and extensively studied AMPs. In this research, I investigated the interaction of these two AMPs with three models of cell membranes: 80% POPC 20% POPG, 40%POPC 40% POPE and 20% POPG, and 80%POPC 20%POPG plus 30% mole fraction of cholesterol. Time-resolved fluorescence emission and fluorescence anisotropy decays of the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) were analyzed to determine the effects of AMPs on the bilayer headgroup packing and changes in the interior of the phospholipid bilayer during the process of pore formation. Time-resolved fluorescence anisotropy of DPH was employed to investigate the dynamics and acyl chain ensemble order in the core of the membrane bilayers. DPH anisotropy decay was interpreted in terms of the Brownian Rotational Diffusion model (BRD). A wide range of molar ratio of peptides to lipids (P/L) 0.33% to 30% was studied and I found that at high P/L> 1/50 DPH fluorescence lifetime decreases with increasing melittin concentration but there was no effect of the magainin 2 on the fluorescence lifetime. The motion of DPH is slowed by adding more melittin as shown by the increasing the rotational correlation lifetime 〈∅〉 of DPH. The anisotropy decay analysis leads to characterization of the order of the phospholipid acyl chains ensemble throughout the depth of the bilayer by calculating the orientational probability distribution function 𝑓(𝜃) sin(𝜃) of DPH. I found the effect of peptide concentration on the interior of the membrane bilayers by calculating the orientational distribution function, 𝑓(𝜃) sin(𝜃), and the rotational correlation time of DPH.
Steady-state fluorescence measurements were also used to study the charge density effect on the peptide bound lipid to vesicles, and it was confirmed that melittin binds to the membrane bilayer from the blue shift of the maximal emission wavelength λmax of the emission of the intrinsic tryptophan residue in melittin. from the blue shift of the maximal emission wavelength λmax of the intrinsic tryptophan residue in melittin.
The results, which were based on three model bilayers, could have significant ramifications for our knowledge of peptides' mechanism of action on more sophisticated model cell membranes with higher physiological significance.
© 2022 Elmukhtar Ehmed Alhatmi
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Alhatmi, Elmukhtar Ehmed, "Effects of Pore-Forming Peptides (Melittin and Magainin 2) on the Phospholipid Bilayer Interior" (2022). Dissertations and Theses. Paper 5914.