A Multiwavelength Digital Holographic Microscope Architecture for Enhancing Life Detection
Sponsor
National Aeronautics & Space Administration (NASA)
Published In
2019 IEEE AEROSPACE CONFERENCE
Document Type
Citation
Publication Date
3-2019
Abstract
Spectral properties of living organism can provide a discriminating method for characterization. However, digital holographic microscopy (DHM), a high-resolution, volumetric imaging technique that is well-suited for examining microbial species is typically monochromatic. Thus, the spectral properties of bacteria are not probed with DHM save only for the wavelength of operation. Here we describe a new DHM instrument which enhances the method via the simultaneous recording of wavelength multiplexed holograms of the sample. This coarse spectral encoding is akin to labeling with RGB values, thereby producing colorized images of the species which enables a new dimension of characterization. This new instrument is derived from a previous architecture which has already demonstrated superior opto/mechanical stability. Herein, this novel instrument is described, and the results of experimental measurements taken with this new instrument are shown.
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Persistent Identifier
https://archives.pdx.edu/ds/psu/30255
Citation Details
Wallace, J. K., Serabyn, E., Lindensmith, C., Nadeau, J., Rider, S., & Bedrossian, M. (2019, March). A multiwavelength digital holographic microscope architecture for enhancing life detection. In 2019 IEEE Aerospace Conference (pp. 1-6). IEEE.
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