Sponsor
The authors acknowledge the support of the National Science Foundation Grant Number 1828793. Portions of this work were supported under a contract from the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration.
Published In
AIMS Biophysics
Document Type
Article
Publication Date
12-2021
Subjects
Digital Microscopy -- Research Analysis
Abstract
Digital holographic microscopy provides the ability to observe throughout a large volume without refocusing. This capability enables simultaneous observations of large numbers of microorganisms swimming in an essentially unconstrained fashion. However, computational tools for tracking large 4D datasets remain lacking. In this paper, we examine the errors introduced by tracking bacterial motion as 2D projections vs. 3D volumes under different circumstances: bacteria free in liquid media and bacteria near a glass surface. We find that while XYZ speeds are generally equal to or larger than XY speeds, they are still within empirical uncertainties. Additionally, when studying dynamic surface behavior, the Z coordinate cannot be neglected.
Rights
© 2021 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
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DOI
10.3934/biophy.2021030
Citation Details
Acres, J., & Nadeau, J. (2021). 2D vs 3D tracking in bacterial motility analysis. AIMS Biophysics, 8(4), 385-399.