Presentation Type

Poster

Start Date

4-5-2022 11:00 AM

End Date

4-5-2022 1:00 PM

Subjects

Asthma, Sensory Nerves, Lungs, In Situ Hybridization

Advisor

Dr. Matthew Drake

Student Level

Undergraduate

Abstract

P2X3 purinoceptors are expressed by airway sensory neurons and activated by extracellular ATP released during periods of cell stress. In asthma, sensitivity to ATP is increased. Airway eosinophilia, which is common in a majority of asthmatics, increases airway sensory nerve density in mice and humans. Whether eosinophils increase neuronal P2X3 expression in asthma is unknown.

P2X3 expression was quantified in vagal ganglia from wild-type (WT) mice and transgenic mice with airway eosinophilia (NJ1726), systemic eosinophilia (NJ1638), and eosinophil-deficiency (PHIL) using RNAscope in situ hybridization. Images were obtained using confocal microscopy. The percentage of P2X3 positive pixels within non-overlapping 50x50 micron sections was quantified using ImageJ. Replicates were averaged to generate a single data point for each animal.

P2X3 RNA was detected in vagal ganglia in all groups. P2X3 was expressed by 52.1 ± 4.0% of neurons in WT mice, 72.9 ± 6.1% in mice with pulmonary eosinophilia, 66.7 ± 5.5% in mice with systemic eosinophilia, and 59.3 ± 4.7% in eosinophil-deficient mice.

P2X3 is highly expressed in vagal sensory neurons and is increased in the setting of chronic eosinophilia, suggesting eosinophils positively regulate sensory nerve purinoceptor expression. Eosinophil effects may contribute to increased ATP sensitivity in humans with eosinophilic asthma.

Persistent Identifier

https://archives.pdx.edu/ds/psu/37470

Included in

Life Sciences Commons

Share

COinS
 
May 4th, 11:00 AM May 4th, 1:00 PM

Effect of Eosinophils in Purinergic Receptor P2X3 Expression in Mouse Sensory Neurons

P2X3 purinoceptors are expressed by airway sensory neurons and activated by extracellular ATP released during periods of cell stress. In asthma, sensitivity to ATP is increased. Airway eosinophilia, which is common in a majority of asthmatics, increases airway sensory nerve density in mice and humans. Whether eosinophils increase neuronal P2X3 expression in asthma is unknown.

P2X3 expression was quantified in vagal ganglia from wild-type (WT) mice and transgenic mice with airway eosinophilia (NJ1726), systemic eosinophilia (NJ1638), and eosinophil-deficiency (PHIL) using RNAscope in situ hybridization. Images were obtained using confocal microscopy. The percentage of P2X3 positive pixels within non-overlapping 50x50 micron sections was quantified using ImageJ. Replicates were averaged to generate a single data point for each animal.

P2X3 RNA was detected in vagal ganglia in all groups. P2X3 was expressed by 52.1 ± 4.0% of neurons in WT mice, 72.9 ± 6.1% in mice with pulmonary eosinophilia, 66.7 ± 5.5% in mice with systemic eosinophilia, and 59.3 ± 4.7% in eosinophil-deficient mice.

P2X3 is highly expressed in vagal sensory neurons and is increased in the setting of chronic eosinophilia, suggesting eosinophils positively regulate sensory nerve purinoceptor expression. Eosinophil effects may contribute to increased ATP sensitivity in humans with eosinophilic asthma.