Presentation Type
Poster
Start Date
5-4-2022 11:00 AM
End Date
5-4-2022 1:00 PM
Subjects
Biology, Ocean, Intertidal, Climate, Physiology
Advisor
Bradley Buckley
Student Level
Masters
Abstract
Marine organisms living within the intertidal zones, such as mussels, experience a wide range of environmental conditions every day. Due to Climate change, these environments are transforming in such a way that may impact a mussels ability to successfully reproduce, grow, and live. Previous studies have shown that successful gamete development in freshwater mussels has been linked to temperature and species behavior has been observed to be influenced by salinity levels. In order to test the impact of temperature and salinity on mussel physiology, mussels were exposed to several treatments with varying conditions. Those conditions included a combination of a temperature (12.8°C ,15.5°C , 18.3°C) and a salinity (0 ppt, 15 ppt, 35ppt). Following the 24 hour treatments, mussels were exposed to air, submerged underwater, or dissected for flow cytometry. The air exposures and water submersions were performed in an effort to understand if the treatments would impact the mussels ability to keep their shells closed and perform other live sustaining tasks. The flow cytometer was used to analyze cells for DNA content, which can show if the cell is in distress. As a result, apoptotic cells contain a fractional DNA content relative to viable cells. This process can be used to look at the health and viability of each individual organ and piece of tissue. Results, at this time, show that following a treatment with low salinity, mussels experience a decrease in muscle mass. Further results are in development.
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Persistent Identifier
https://archives.pdx.edu/ds/psu/37481
Included in
How are Changes in Temperature and Salinity Impacting Intertidal Mussels?
Marine organisms living within the intertidal zones, such as mussels, experience a wide range of environmental conditions every day. Due to Climate change, these environments are transforming in such a way that may impact a mussels ability to successfully reproduce, grow, and live. Previous studies have shown that successful gamete development in freshwater mussels has been linked to temperature and species behavior has been observed to be influenced by salinity levels. In order to test the impact of temperature and salinity on mussel physiology, mussels were exposed to several treatments with varying conditions. Those conditions included a combination of a temperature (12.8°C ,15.5°C , 18.3°C) and a salinity (0 ppt, 15 ppt, 35ppt). Following the 24 hour treatments, mussels were exposed to air, submerged underwater, or dissected for flow cytometry. The air exposures and water submersions were performed in an effort to understand if the treatments would impact the mussels ability to keep their shells closed and perform other live sustaining tasks. The flow cytometer was used to analyze cells for DNA content, which can show if the cell is in distress. As a result, apoptotic cells contain a fractional DNA content relative to viable cells. This process can be used to look at the health and viability of each individual organ and piece of tissue. Results, at this time, show that following a treatment with low salinity, mussels experience a decrease in muscle mass. Further results are in development.